Hewson Lab at Cornell

Team Aquatic Virus - Microbial Oceanography, P.I. Ian Hewson, Ph.D.

Team Aquatic Virus Lab Protocols

Preparation of Metatranscriptomic RNA

  1. in vitro transcription – Ambion MessageAmp II - Bacteria
  2. Conversion of aRNA to double stranded cDNA
  3. ds cDNA clean-up for Pyrosequencing

Preparation of DNA and RNA Viriomes

  1. Filter Chloroform Nuclease Virus Purification Protocol

Pico Green Quantification of DNA by qPCR Machine

  1. Hewson Lab DNA Quantification protocol